| 质粒名称: | pT7-PE7 for IVT |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 214813 |
| 存储实验室: | Brittany Adamson |
|
载体骨架基本信息 |
|
| 载体名称: | pT7 |
| 载体出品公司: | Clontech |
| 空载体大小: | 3481bp |
| 完整质粒大小: | 10552bp |
| 载体类型: | Template for in vitro transcription |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PE7 / PEmax-La(SSB)1-194 |
| 物种来源: | Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus; La(SSB)1-194 is from Homo sapiens |
| 插入大小: | 7071bp |
| 启动子: | T7 (inactivated) |
| 标签/融合蛋白: |
SV40 bpNLS (N terminal on insert)
SV40 bpNLS (C terminal on insert)
c-Myc NLS (C terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | M13 Reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pT7-PE7 for IVT was a gift from Brittany Adamson (Addgene plasmid # 214813 ; http://n2t.net/addgene:214813 ; RRID:Addgene_214813) |
| 参考文献部分: | Improving prime editing with an endogenous small RNA-binding protein. Yan J, Oyler-Castrillo P, Ravisankar P, Ward CC, Levesque S, Jing Y, Simpson D, Zhao A, Li H, Yan W, Goudy L, Schmidt R, Solley SC, Gilbert LA, Chan MM, Bauer DE, Marson A, Parsons LR, Adamson B. Nature. 2024 Apr 3. doi: 10.1038/s41586-024-07259-6. 10.1038/s41586-024-07259-6 PubMed 38570691 |
买家导航
pT7-PE7 for IVT载体质粒基本信息
pT7-PE7 for IVT质粒图谱载体图谱和pT7-PE7 for IVT载体序列质粒序列多克隆位点信息
pT7-PE7 for IVT质粒载体简介
pT7-PE7 for IVT质粒序列载体序列
LOCUS pT7-PE7 for IVT 10552 bp ds-DNA circular SYN 20-MAR-2024
DEFINITION Template for in vitro transcription of PE7.
ACCESSION .
VERSION .
KEYWORDS pT7-PE7 for IVT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10552)
TITLE An endogenous small RNA-binding protein safeguards prime editing
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10552)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 20, 2024 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10552
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..204
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 155..175
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 340..360
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 361..4461
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 4519..4539
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7318..7338
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7348..7374
/codon_start=1
/product="nuclear localization signal of human c-Myc
proto-oncogene (Dang and Lee, 1988)"
/label=c-myc NLS
/translation="PAAKRVKLD"
polyA_signal 7482..7706
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(7777..7793)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7777..7793)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7790..7812)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7801..7817
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7825..7855)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7870..7891
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8008..8025)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8179..8767)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8259..8278)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8938..9798)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9561..9580
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9799..9903)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9982..10001)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 10173..10552
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatctcgag ctcggtacct
241 aatacgacac actataagga aataagagag aaaagaagag taagaagaaa tataagagcc
301 accatgaaac ggacagccga cggaagcgag ttcgagtcac caaagaagaa gcggaaagtc
361 gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc
421 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
481 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
541 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
601 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
661 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
721 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
781 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
841 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
901 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
961 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagaaag
1021 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
1081 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
1141 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
1201 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
1261 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
1321 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
1381 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
1441 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
1501 aagatggacg gcaccgagga actgctcgtg aagctgaaga gagaggacct gctgcggaag
1561 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
1621 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
1681 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
1741 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
1801 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
1861 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
1921 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
1981 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
2041 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
2101 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
2161 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
2221 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
2281 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
2341 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
2401 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
2461 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
2521 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
2581 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
2641 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
2701 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
2761 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
2821 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc
2881 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
2941 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
3001 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
3061 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
3121 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
3181 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
3241 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
3301 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
3361 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
3421 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
3481 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
3541 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
3601 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
3661 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
3721 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
3781 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
3841 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
3901 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
3961 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
4021 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
4081 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
4141 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
4201 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
4261 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
4321 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
4381 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
4441 ctgtctcagc tgggaggtga ctccggcgga agctctggtg gcagcaagcg gaccgccgac
4501 ggctctgaat tcgagagccc taagaagaaa agaaaggtga gcggaggctc tagcggcgga
4561 agcaccctga acattgaaga cgagtataga ctgcatgaaa caagcaagga acccgatgtg
4621 tccctgggct ccacctggct gtccgacttt ccccaggcct gggccgagac aggaggaatg
4681 ggcctggccg tgcggcaggc acccctgatc atccctctga aggccacctc tacacccgtg
4741 agcatcaagc agtaccctat gtctcaggag gccagactgg gcatcaagcc tcacatccag
4801 aggctgctgg accagggcat cctggtgcca tgccagagcc cctggaacac accactgctg
4861 cccgtgaaga agccaggcac caatgactat agacccgtgc aggatctgag agaggtgaac
4921 aagagggtgg aggatatcca ccccaccgtg cccaaccctt acaatctgct gtccggcctg
4981 cccccttctc accagtggta tacagtgctg gacctgaagg atgccttctt ttgtctgaga
5041 ctgcacccta ccagccagcc actgttcgcc tttgagtgga gggaccctga gatgggcatc
5101 tctggccagc tgacctggac acgcctgcct cagggcttca agaatagccc aacactgttt
5161 aacgaggccc tgcaccgcga cctggcagat ttccggatcc agcacccaga tctgatcctg
5221 ctgcagtacg tggacgatct gctgctggcc gccaccagcg agctggattg ccagcaggga
5281 acacgcgccc tgctgcagac cctgggaaac ctgggatata gggcatccgc caagaaggcc
5341 cagatctgtc agaagcaggt gaagtacctg ggctatctgc tgaaggaggg ccagagatgg
5401 ctgacagagg ccaggaagga gacagtgatg ggccagccaa cacccaagac cccaagacag
5461 ctgagggagt tcctgggcaa agcaggattt tgcaggctgt tcatcccagg attcgcagag
5521 atggcagcac ctctgtaccc actgaccaag ccgggcaccc tgtttaattg gggccctgac
5581 cagcagaagg cctatcagga gatcaagcag gccctgctga cagcaccagc cctgggcctg
5641 ccagacctga ccaagccttt cgagctgttt gtggatgaga agcagggcta cgccaagggc
5701 gtgctgaccc agaagctggg accatggaga cggcccgtgg cctatctgtc caagaagctg
5761 gacccagtgg cagcaggatg gccaccatgc ctgaggatgg tggcagcaat cgccgtgctg
5821 acaaaggatg ccggcaagct gaccatggga cagccactgg tcatcctggc accacacgca
5881 gtggaggccc tggtgaagca gcctccagat cgctggctgt ctaacgcccg gatgacacac
5941 taccaggccc tgctgctgga caccgatcgc gtgcagtttg gccctgtggt ggccctgaat
6001 ccagccaccc tgctgcctct gccagaggag ggcctgcagc acaactgtct ggacatcctg
6061 gcagaggcac acggaacaag gccagacctg accgatcagc ccctgcctga cgccgatcac
6121 acatggtata ccgatggaag ctccctgctg caggagggcc agaggaaggc aggagcagca
6181 gtgaccacag agacagaagt gatctgggcc aaggccctgc cagcaggcac atccgcccag
6241 cgggccgagc tgatcgccct gacccaggcc ctgaagatgg ccgagggcaa gaagctgaac
6301 gtgtacacag actccagata tgccttcgcc accgcacaca tccacggaga gatctacagg
6361 cgccggggct ggctgacctc tgagggcaag gagatcaaga acaaggatga gatcctggcc
6421 ctgctgaagg ccctgtttct gcccaagcgg ctgagcatca tccactgtcc tggacaccag
6481 aagggacact ccgccgaggc aaggggcaat cggatggccg accaggccgc cagaaaggct
6541 gctattactg aaactcccga cacttccact ctgctgattg aaaactcctc cccttccgga
6601 ggatctagcg gaggctcctc tggctctgag acacctggca caagcgagag cgcaacacct
6661 gaaagcagcg ggggcagcag cggggggtca atggctgaaa atggtgataa tgaaaagatg
6721 gctgccctgg aggccaaaat ctgtcatcaa attgagtatt attttggcga cttcaatttg
6781 ccacgggaca agtttctaaa ggaacagata aaactggatg aaggctgggt acctttggag
6841 ataatgataa aattcaacag gttgaaccgt ctaacaacag actttaatgt aattgtggaa
6901 gcattgagca aatccaaggc agaactcatg gaaatcagtg aagataaaac taaaatcaga
6961 aggtctccaa gcaaacccct acctgaagtg actgatgagt ataaaaatga tgtaaaaaac
7021 agatctgttt atattaaagg cttcccaact gatgcaactc ttgatgacat aaaagaatgg
7081 ttagaagata aaggtcaagt actaaatatt cagatgagaa gaacattgca taaagcattt
7141 aagggatcaa tttttgttgt gtttgatagc attgaatctg ctaagaaatt tgtagagacc
7201 cctggccaga agtacaaaga aacagacctg ctaatacttt tcaaggacga ttactttgcc
7261 aaaaaaaatg aatctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagtctccc
7321 aagaagaaga ggaaagtcgg ctctggccct gccgctaaga gagtgaagct ggactaatta
7381 attaagctgc cttctgcggg gcttgccttc tggccatgcc cttcttctct cccttgcacc
7441 tgtacctctt ggtctttgaa taaagcctga gtaggaagcg actgtgcctt ctagttgcca
7501 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
7561 tgtcctttcc taataaaatg agaaaattgc atcgcattgt ctgagtaggt gtcattctat
7621 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
7681 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat
7741 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
7801 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagccta
7861 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
7921 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
7981 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
8041 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
8101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
8161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
8221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
8281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
8341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
8401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
8461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
8521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
8581 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
8641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
8701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
8761 atctcaagaa gatcctttga tcttttctac ggggtctgac actcagtgga acgaaaactc
8821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
8881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
8941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
9001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
9061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
9121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
9181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
9241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
9301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
9361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
9421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
9481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
9541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
9601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
9661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
9721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
9781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
9841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
9901 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc gatctcccga
9961 tcccctaggg tctactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
10021 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
10081 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
10141 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
10201 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
10261 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
10321 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
10381 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
10441 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
10501 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tg
//
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pT7-PE7 for IVT质粒载体应用举例
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